《植物生理学报》 2010, 46(8): 787-792
通信作者:周开兵;E-mail: kaibingzhou0528@163.com;Tel: 0898-66182916
摘 要:
以‘ 台农一号’ 芒果盆栽苗离体老叶为试材, 研究增强UV-B 辐射条件下芒果老叶的损伤和保护反应。结果表明: UV- B 辐射处理使芒果叶片MDA 含量和相对电导率升高、叶绿素含量和叶绿素a/b 降低, 表明叶片受到损伤, 且随处理时间延 长叶片损伤加重。UV-B 辐射处理叶片可溶性蛋白含量、抗氧化酶(SOD、CAT、POD)活性、保护色素(类胡萝卜素、类黄 酮)和还原型 GSH 含量显著高于对照叶片, UV-B 辐射处理叶片维生素 C 含量显著低于对照叶片, 表明增强UV-B 辐射可诱 导叶片细胞通过提高活性氧清除能力和积累保护色素而直接吸收部分UV-B辐射来提高抗增强UV-B辐射损伤的能力。关键词:增强UV-B辐射; 芒果; 老叶; 损伤; 保护反应
收稿:2010-02-02 修定:2010-05-06
资助:海南省自然科学基金项目(807005)。
Corresponding author: ZHOU Kai-Bing; E-mail: kaibingzhou0528@163.com; Tel: 0898-66182916
Abstract:
Old leaves of mango potted young trees were used to study damages and protection response under the enhanced UV-B radiation. The results showed that after UV-B treatment the content of MDA and the relative conductivity were higher, while the content of chlorophyll and the ratio of chlorophyll a/b became lower. It indicated the enhanced UV-B radiation caused the damages of leaves, which would be worse with longer treatment period. The UV-B treated leaves had higher contents of soluble protein, protective pigments (flavonodis, carotenoids) and reducd GSH, higher activities of antioxidant enzymes (SOD, CAT and POD), and lower content of vitamin C than CK. It indicated the enhanced UV-B radiation induced elevated resistance of enhanced UVB radiation in cells through intensifying the oxidant scavenging activities and absorbing part of the enhanced UVB radiation.Key words: enhanced UV-B radiation; mango (Mangifera indica); old leaf; damage; protection response
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